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Fluor	Comment	EX(nm)	EM(nm)	WideField Sets			Laser Sets
A
Acridine Orange + DNA	IF workers must detect fluorescence from both RNA and DNA-bound Acridine Orange a longpass set such as 49012 is recommnded. 49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.	500	526	49011	49002	39002
Acridine Orange + DNA and RNA	49012 may be used with LEDs with CWL 465-495nm; 19002 may be used with 470-490nm CWL LEDs.			49012	19002
Acridine Orange + RNA	39009 may be used with LEDs with CWL 470-490nm; 49024 may be used with 430-450nm CWL LEDs.	460	650	39009	49024
Alexa Fluor 350	49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.	346	442	49000	39000		49915
Alexa Fluor 488	49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.	498	520	49011	49002	39002	49904
Alexa Fluor 514	49003, 39003 may be used with LEDs with CWL 490-505nm; 49023 may be use with 485-515nm CWL LEDs.	517	539	49003	49023	39003	49905	49906
Alexa Fluor 532	49014 may be used with LEDs with CWL520-540nm.	531	554	49014			49907	49906
Alexa Fluor 546	49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs	556	573	49004	49305	39004	49909	49908
Alexa Fluor 555	49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.	553	568	49004	49305	39004	49907	49908
Alexa Fluor 568	49008, 39010 may be used with LEDs with CWL 545-575nm; 49305 may be used with 535-550 CWL LEDs.	578	603	49008	49005	39010	49910	49909
Alexa Fluor 594	49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.	590	617	49008	39010	49306	49911
Alexa Fluor 633	49015 may be used with LEDs with CWL 585-620nm.	632	647	49015			49913
Alexa Fluor 647	49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.	649	666	49006	49009	39007	49913	49914
Alexa Fluor 680	49022 may be used with LEDs with CWL 635-665nm.	680	702	49022			49914
Alexa Fluor 700	49022 may be used with LEDs with CWL 635-665nm.	696	719	49022			49913	49914
Alexa Fluor 750	49007 may be used with LEDs with CWL 685-740nm.	752	779	49007
Alexa Fluor 790	49037 may be used with LEDs with CWL 725-755nm.	782	805	49037
Allophycocyanin (APC)	49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs	630	660	49006	39007		49913	49914
AmCyan	49001, 39001 may be used with LEDs with CWL 430-440nm.	458	489	49001	39001		49903	49902
AsRed2	49005, 49305 may be used with LEDs with CWL 535-555nm.	576	592	49005	49305		49909	49910
Atto 390	49028 may be used with LEDs with CWL385-405nm.	390	479	49028
Atto 425	49013 may be used with LEDs with CWL 435-455nm; 39001 may be used with 430-440nm CWL LEDs.	436	485	49013	39001		49903	49902
Atto 465	49002 may be used with LEDs with CWL 455-485nm.	453	507	49002			49903
Atto 488	49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.	501	525	49011	39002	49003	49904
Atto 550	49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.	554	576	49004	39004		49909	49908
Atto 633	49015 may be used with LEDs with CWL 585-620nm.	630	657	49015			49913
Atto 647N	49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.	644	669	49006	49009	39007	49913	49914
Atto 680	49022 may be used with LEDs with CWL 635-665nm	680	700	49022			49914
Auramine	19008 may be used with LEDs with CWL 435-470nm.	450	500	19008
Auramine-rhodamine	19002 may be used with LEDs with CWL 470-490nm.	450	500-600	19002
Azami Green	49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.	492	505	49002	49020	39002	49904
B
BCECF/pH 5.2		482	520	79005
BCECF/pH 9.0		503	528	79005
BODIPY FL/pH7.2	49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED	505	512	49011	49002	39002	49904
C
Calcein	49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.	494	517	49011	49003	39002	49904
Calcium Green-1	49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.	506	531	49011	49003	39002	49904
Calcofluor White	Calcofluor White is a commonly used stain to detect fungi and yeast and other microorganisms. It is a non-specific stain which preferentially binds to chitin and cellulose in cell walls and as such is also used to visualize plant cells. Calcofluor White has extremely broad excitation/emission spectra, and as a result almost any filter set with UV or violet/blue excitation wavelengths will generate fluorescence emission which may be detected across a very broad range. Some samples will fluoresce strongly in the green wavelengths, others in the blue, and others yellow-orange. Because of this, filter selection is subjective, partly depending on the experience of the microscopist. Our filter sets 19012, 19000, and 19011 may be used successfully depending on the sample and the observer's preference. 19000 and 19012 may be used with LEDs with CWL 365-380nm; 19011 may be used with 395-410nm CWL LEDs.	350	450-500	19012	19000
Cerulean	49001, 39001 may be used with LEDs with CWL 430-440nm.	433	475	49001	39001		49903	49902
CFP	49001, 39001 may be used with LEDs with CWL 430-440nm.	433	475	49001	39001		49903	49902
Citrine	49003, 39003 may be used with LEDs with CWL 490-505nm.	514	529	49003	39003		49905	49904
Coumarin	49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs, 19011 may be used with 395-410nm CWL LEDs.	384	470	49000	39000	19011
Cy2	49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.	489	506	49011	49002	39002	49904
Cy3.5	49008, 39010 may be used with LEDs with CWL 545-575nm; 49305 may be used with 535-550 CWL LEDs.	581	598	49008	49305	39010	49910	49909
Cy3	49004, 49005 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.	552	570	49004	49005	39004	49907	49908
Cy5.5	49022 may be used with LEDs with CWL 635-665nm.	675	695	49022			49914
Cy5	49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.	649	670	49006	39007	49009	49913	49914
Cy7	49007 may be used with LEDs with CWL 685-740nm.	743	767	49007
D
DAPI	49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs, 49028 may be used with 385-400nm CWL LEDs.	359	461	49000	39000	49028
Di-8-ANEPPS non-ratiometric		530	620	39009
Di-8-ANEPPS, ratiometric	This dye and the related dye, Di-4-ANEPPS, are most effectively used as ratiometric indicators of membrane potential. Increases in membrane potential are indicated by a decrease in fluorescence intensity with approx. 440nm excitation and an increase in fluorescence intensity with approx. 530nm excitation. Use of this 71006 filter set requires an external filter wheel to house the 2 excitation filters and to facilitate rapid switching of filters for ratiometric measurements.			71006
DiA	49024 may be used with LEDs with CWL 425-455nm; 39009, 19010 may be used with 470-490nm CWL LEDs.	456	591	49024	39009	19010
DiD	49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.	644	665	49006	39007		49913	49914
DiI	49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.	551	569	49004	39004	49305	49908	49909
DiO	49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.	484	502	49002	39002	49020	49904
DiR	49007 may be used with LEDs with CWL 685-740nm.	748	780	49007
Draq5	49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.	647	683	49006	39007		49913	49914
DsRed	49004 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.	557	584	49004	39004		49909	49908
DyLight 350	49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.	352	435	49000	39000		49915
DyLight 405	49021 may be used with LEDs with CWL 400-410nm.	399	433	49021	19011		49901
DyLight 488	49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.	492	517	49011	39002	49002	49904
DyLight 549	49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.	554	569	49004	39004	49305	49907	49909
DyLight 594	49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.	592	616	49008	39010	49306	49911
DyLight 633	49015 may be used with LEDs with CWL 585-620nm.	623	645	49015
DyLight 649	49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.	652	667	49006	39007	49009	49913	49914
DyLight 680	49022 may be used with LEDs with CWL 635-665nm.	677	705	49022
DyLight 750	49007 may be used with LEDs with CWL 685-740nm.	751	772	49007
DyLight 800	49037 may be used with LEDs with CWL 725-755nm.	770	795	49037
E
EBFP2	49028 may be used with LEDs with CWL 385-400nm, 49021 may be used with 400-410nm CWL LEDs.	385	448	49021	49028		49901
ECFP	49001, 39001 may be used with LEDs with CWL 430-440nm.	434	477	49001	39001		49902	49903
EGFP	49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.	488	507	49002	49020	39002	49904
Emerald GFP	49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.	489	510	49002	49020	39002	49904
Eosin	49023 may be used with LEDs with CWL 490-520nm.	524	545	49023
Ethidium Bromide	49005 may be used with LEDs with CWL 535-555nm; 39009 may be used with 470-490nm CWL LEDs.	520	603	49005	39009
Ethidium homidimer-1/DNA	49005 may be used with LEDs with CWL 535-555nm; 39005 may be used with 535-550nm CWL LEDs.	527	617	49005	39005
EYFP/pH 7	49003, 39003 may be used with LEDs with CWL 490-505nm	514	527	49003	39003
F
FAM	49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.	492	518	49011	49002	39002
FITC	49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.	490	525	49011	49002	39002
FlAsH-CCPFCC	49003, 39003 may be used with LEDs with CWL 490-505nm.	511	530	49003	39003
Fluo-3	49003 may be used with LEDs with CWL 490-505nm; 49011 may be used with 465-495nm CWL LEDs.	505	526	49003	49011
Fluo-4	49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.	494	516	49011	49002	39002
FluoroGold	This dye has mainly been used as a retrograde neuronal tracer, although it can also distinguish between DNA and RNA binding according to its fluorescence emission. With two excitation peaks at approx. 330nm and 390nm, and two emission peaks at approx. 450nm and 600nm, our DAPI longpass filter sets 49025 and 19012 are appropriate for detection of the fluorescence emission associated with the both peaks, which together appear "gold". The 49025 set is also useful for distinguishing between FluoroGold bound to DNA or RNA.			49025	19012
FM 1-43	39009, 19010 may be used with LEDs with CWL 470-490nm.	479	598	39009	19010
FM 4-64	For imaging applications using a monochrome camera, this longpass filter set will not distinguish FM 4-64 from TRITC or similar orange/red fluorochromes. To be able to distinguish between FM 4-64 and TRITC or Texas Red-like fluorochromes, for a custom filter set. 19010 may be used with LEDs with CWL 470-490nm; 19006 may be used with 545-575nm CWL LEDs.	515	740	19010	19006
Fura-2/Ca2+ - free		340	510	79001
Fura-2/Ca2+ - saturated		380	510	79001
FusionRed	49008, 39010 and 49017 may be used with LEDs with CWL 545-575nm.	576	603	49008	49017	39010	49910	49909
G
GFP	EGFP spectra used here; similar to Emerald GFP, TagGFP, Azami Green, mWasabi, etc.49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.	488	507	49002	49020	39002
H
HEX,SE	49014 may be used with LEDs with CWL520-540nm; 49023 may be use with 485-515nm CWL LEDs.	533	559	49014	41023
Hoechst 33258	49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.	352	461	49000	39000
I
Indo-1/Ca2+ -free	79006 may be used with LEDs with CWL 365nm.	346	475	79006
indo-1/Ca2+ -saturated	79006 may be used with LEDs with CWL 365nm.	330	401	79006
Indocyanine Green	The spectral properties of ICG are strongly dependent on its concentration and the solute it is bound to, so care must be taken by workers to characterize their particular model system. This filter set 49030 is appropriate for most applications involving protein-bound ICG in various physiological media. ICG becomes concentrated in lipid environments, greatly shifting its spectral properties. 49030 may be used with LEDs with CWL 755-795nm.			49030
J
JC-1 non-ratiometric	JC-1 exists as a monomer at low concentrations and/or low membrane potentials and displays fluorescence ex./em. maxima similar to that of YFP. At higher concentrations and/or higher membrane potentials, JC-1 forms aggregates with a red-shifted emission maxima of 590nm and a broadened excitation spectra. The 49012 longpass filter set will allow detection of JC-1 monomer and aggregate simultaneously.			49012	19002
JOE	49023 may be use with LEDs with CWL 485-515nm.	520	548	49023			49905
K
Killer Red	49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.	585	611	49008	39010	49306	49911	49910
L
Li-Cor IRDye 680LT	49022 may be used with LEDs with CWL 635-665nm.	674	693	49022
Li-Cor IRDye 800CW	49037 may be used with LEDs with CWL 725-755nm.	778	794	49037
Lucifer Yellow	49026 may be used with LEDs with CWL 390-415nm; 39008 may be used with 405-430nm CWL LEDs	428	540	39008	49026
LysoTracker Blue/MeOH	49028 may be used with LEDs with CWL 385-400nm; 49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.	373	425	49000	39000	49028
LysoTracker Green/pH 5.2		504	511	49002	49020	39002	49904
LysoTracker Red/pH 5.2		577	590	49008			49909
LysoTracker Yellow HCK-123		486	563	39009	49023
M
mCherry		587	610	49008	49306	39010	49910	49911
mCitrine	49003, 39003 may be used with LEDs with CWL 490-505nm; 49023 may be use with 490-520nm CWL LEDs.	514	529	49003	39003		49905	49904
MitoTracker Deep Red 633/MeOH		644	665	49006	49009	39007
MitoTracker Green FM/MeOH		490	516	49002	49011	39002
MitoTracker Orange/MeOH		551	576	49004	49305	39004
MitoTracker Red/MeOH		578	599	49008	49306	39010	49910
mKate2		589	634	49008	39010	49306	49911	49912
mKeima Red		440	620	49024
mKO		548	559	49010	49014		49907	49906
mOrange2		548	562	49010	49014		49907	49906
mRFP1		584	607	49008	39010	49306	49910	49911
mTFP1		462	492	49013			49903	49902
mWasabi		493	509	49002	49020	39002	49904
N
NBD X/MeOH		467	538	49011	19008
Nile Blue		631	660	49006	39007
NirFP		604	669	49019			49912
O
Oregon Green 488		490	514	49011	49003	39002
Oregon Green 514	49003, 39003 may be used with LEDs with CWL 490-505nm.	506	526	49003	49303	39003
P
Pacific Blue		405	455	49021	19011
Propidium Iodide		536	617	49005	39005
Q
Qdot 525	Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.	250-450	525	39101
Qdot 545	Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.	250-450	545	39102
Qdot 565	Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.	250-450	565	39103
Qdot 585	Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.	250-450	585	39104
Qdot 605	Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.	250-450	605	39105
Qdot 625	Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.	250-450	625	39106
Qdot 655	Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.	250-450	655	39107
Qdot 705	Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.	250-450	705	39108
Qdot 800	Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.	250-450	800	39109
R
R-phycoerythrin		565	578	49010	49014	19004	49909
ReAsH-CCPGCC		592	606	49008	39010	49306
Rhod-2	49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.	552	581	49004	49305	39004	49908
Rhodamine 123	49003, 39003 may be used with LEDs with CWL 490-505nm.	507	529	49003	39003		49905
Rhodamine 6G	49023 may be use with LEDs with CWL 485-515nm.	525	555	49023			49906	49907
Rhodamine Red-X		570	590	49008		39010	49910
ROX		578	604	49008	39010		49910
S
SBFI/Na+ -free		340	505	79001
SBFI/Na+ -saturated		380	505	79001
Sulforhodamine 101		586	605	49008	39010
SYBR Green I		498	522	49002	49011	39002	49904
SYTO 9/DNA		483	500	49002	49011	39002	49904
SYTO 60		652	678	49006	49009	39007	49913	49914
T
TagBFP	49021 may be used with 400-410nm CWL LEDs, 49028 may be used with 385-400nm CWL LEDs	402	457	49021		49028	49901
TagRFP		556	584	49004	39004	49305	49909	49908
TAMRA		555	580	49004	49005	39004	49909
tdTomato		554	581	49004	49305	39004	49909	49908
TET		521	536	49023
Tetramethylrhodamine isothiocyanate		555	580	49004	49305	39004	49909	49908
Texas Red		595	620	49008	39010	49306	49911	49910
Texas Red-X		595	615	49008	39010	49306
TO-PRO-3		642	661	49006	39007	49009
Topaz	49003, 39003 may be used with LEDs with CWL 490-505nm.	514	529	49003	39003		49905	49904
TRITC		555	580	49004	49305	39004	49909	49908
TurboFP650		592	650	49008	19006	49017	49912	49911
W
wtGFP		475	509	49002		19008
X
X-rhod-1/Ca2+		580	601	49008	39010	49306
Z
ZsGreen1		493	505	49002	49020	39002	49904
ZsYellow		529	539	49023	49003		49905
				39004	49305



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