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Fluor Comment
EX(nm)
EM(nm)
WideField Sets
Laser Sets
A
Acridine Orange + DNA IF workers must detect fluorescence from both RNA and DNA-bound Acridine Orange a longpass set such as 49012 is recommnded. 49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.
500
526
49011
49002
39002
Acridine Orange + DNA and RNA 49012 may be used with LEDs with CWL 465-495nm; 19002 may be used with 470-490nm CWL LEDs.
49012
19002
Acridine Orange + RNA 39009 may be used with LEDs with CWL 470-490nm; 49024 may be used with 430-450nm CWL LEDs.
460
650
39009
49024
Alexa Fluor 350 49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.
346
442
49000
39000
49915
Alexa Fluor 488 49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.
498
520
49011
49002
39002
49904
Alexa Fluor 514 49003, 39003 may be used with LEDs with CWL 490-505nm; 49023 may be use with 485-515nm CWL LEDs.
517
539
49003
49023
39003
49905
49906
Alexa Fluor 532 49014 may be used with LEDs with CWL520-540nm.
531
554
49014
49907
49906
Alexa Fluor 546 49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs
556
573
49004
49305
39004
49909
49908
Alexa Fluor 555 49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
553
568
49004
49305
39004
49907
49908
Alexa Fluor 568 49008, 39010 may be used with LEDs with CWL 545-575nm; 49305 may be used with 535-550 CWL LEDs.
578
603
49008
49005
39010
49910
49909
Alexa Fluor 594 49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.
590
617
49008
39010
49306
49911
Alexa Fluor 633 49015 may be used with LEDs with CWL 585-620nm.
632
647
49015
49913
Alexa Fluor 647 49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.
649
666
49006
49009
39007
49913
49914
Alexa Fluor 680 49022 may be used with LEDs with CWL 635-665nm.
680
702
49022
49914
Alexa Fluor 700 49022 may be used with LEDs with CWL 635-665nm.
696
719
49022
49913
49914
Alexa Fluor 750 49007 may be used with LEDs with CWL 685-740nm.
752
779
49007
Alexa Fluor 790 49037 may be used with LEDs with CWL 725-755nm.
782
805
49037
Allophycocyanin (APC) 49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs
630
660
49006
39007
49913
49914
AmCyan 49001, 39001 may be used with LEDs with CWL 430-440nm.
458
489
49001
39001
49903
49902
AsRed2 49005, 49305 may be used with LEDs with CWL 535-555nm.
576
592
49005
49305
49909
49910
Atto 390 49028 may be used with LEDs with CWL385-405nm.
390
479
49028
Atto 425 49013 may be used with LEDs with CWL 435-455nm; 39001 may be used with 430-440nm CWL LEDs.
436
485
49013
39001
49903
49902
Atto 465 49002 may be used with LEDs with CWL 455-485nm.
453
507
49002
49903
Atto 488 49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.
501
525
49011
39002
49003
49904
Atto 550 49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
554
576
49004
39004
49909
49908
Atto 633 49015 may be used with LEDs with CWL 585-620nm.
630
657
49015
49913
Atto 647N 49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.
644
669
49006
49009
39007
49913
49914
Atto 680 49022 may be used with LEDs with CWL 635-665nm
680
700
49022
49914
Auramine 19008 may be used with LEDs with CWL 435-470nm.
450
500
19008
Auramine-rhodamine 19002 may be used with LEDs with CWL 470-490nm.
450
500-600
19002
Azami Green 49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.
492
505
49002
49020
39002
49904
B
BCECF/pH 5.2
482
520
79005
BCECF/pH 9.0
503
528
79005
BODIPY FL/pH7.2 49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED
505
512
49011
49002
39002
49904
C
Calcein 49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.
494
517
49011
49003
39002
49904
Calcium Green-1 49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.
506
531
49011
49003
39002
49904
Calcofluor White Calcofluor White is a commonly used stain to detect fungi and yeast and other microorganisms. It is a non-specific stain which preferentially binds to chitin and cellulose in cell walls and as such is also used to visualize plant cells. Calcofluor White has extremely broad excitation/emission spectra, and as a result almost any filter set with UV or violet/blue excitation wavelengths will generate fluorescence emission which may be detected across a very broad range. Some samples will fluoresce strongly in the green wavelengths, others in the blue, and others yellow-orange. Because of this, filter selection is subjective, partly depending on the experience of the microscopist. Our filter sets 19012, 19000, and 19011 may be used successfully depending on the sample and the observer's preference. 19000 and 19012 may be used with LEDs with CWL 365-380nm; 19011 may be used with 395-410nm CWL LEDs.
350
450-500
19012
19000
Cerulean 49001, 39001 may be used with LEDs with CWL 430-440nm.
433
475
49001
39001
49903
49902
CFP 49001, 39001 may be used with LEDs with CWL 430-440nm.
433
475
49001
39001
49903
49902
Citrine 49003, 39003 may be used with LEDs with CWL 490-505nm.
514
529
49003
39003
49905
49904
Coumarin 49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs, 19011 may be used with 395-410nm CWL LEDs.
384
470
49000
39000
19011
Cy2 49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.
489
506
49011
49002
39002
49904
Cy3.5 49008, 39010 may be used with LEDs with CWL 545-575nm; 49305 may be used with 535-550 CWL LEDs.
581
598
49008
49305
39010
49910
49909
Cy3 49004, 49005 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
552
570
49004
49005
39004
49907
49908
Cy5.5 49022 may be used with LEDs with CWL 635-665nm.
675
695
49022
49914
Cy5 49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.
649
670
49006
39007
49009
49913
49914
Cy7 49007 may be used with LEDs with CWL 685-740nm.
743
767
49007
D
DAPI 49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs, 49028 may be used with 385-400nm CWL LEDs.
359
461
49000
39000
49028
Di-8-ANEPPS non-ratiometric
530
620
39009
Di-8-ANEPPS, ratiometric This dye and the related dye, Di-4-ANEPPS, are most effectively used as ratiometric indicators of membrane potential. Increases in membrane potential are indicated by a decrease in fluorescence intensity with approx. 440nm excitation and an increase in fluorescence intensity with approx. 530nm excitation. Use of this 71006 filter set requires an external filter wheel to house the 2 excitation filters and to facilitate rapid switching of filters for ratiometric measurements.
71006
DiA 49024 may be used with LEDs with CWL 425-455nm; 39009, 19010 may be used with 470-490nm CWL LEDs.
456
591
49024
39009
19010
DiD 49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.
644
665
49006
39007
49913
49914
DiI 49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
551
569
49004
39004
49305
49908
49909
DiO 49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.
484
502
49002
39002
49020
49904
DiR 49007 may be used with LEDs with CWL 685-740nm.
748
780
49007
Draq5 49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.
647
683
49006
39007
49913
49914
DsRed 49004 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
557
584
49004
39004
49909
49908
DyLight 350 49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.
352
435
49000
39000
49915
DyLight 405 49021 may be used with LEDs with CWL 400-410nm.
399
433
49021
19011
49901
DyLight 488 49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.
492
517
49011
39002
49002
49904
DyLight 549 49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
554
569
49004
39004
49305
49907
49909
DyLight 594 49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.
592
616
49008
39010
49306
49911
DyLight 633 49015 may be used with LEDs with CWL 585-620nm.
623
645
49015
DyLight 649 49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.
652
667
49006
39007
49009
49913
49914
DyLight 680 49022 may be used with LEDs with CWL 635-665nm.
677
705
49022
DyLight 750 49007 may be used with LEDs with CWL 685-740nm.
751
772
49007
DyLight 800 49037 may be used with LEDs with CWL 725-755nm.
770
795
49037
E
EBFP2 49028 may be used with LEDs with CWL 385-400nm, 49021 may be used with 400-410nm CWL LEDs.
385
448
49021
49028
49901
ECFP 49001, 39001 may be used with LEDs with CWL 430-440nm.
434
477
49001
39001
49902
49903
EGFP 49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.
488
507
49002
49020
39002
49904
Emerald GFP 49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.
489
510
49002
49020
39002
49904
Eosin 49023 may be used with LEDs with CWL 490-520nm.
524
545
49023
Ethidium Bromide 49005 may be used with LEDs with CWL 535-555nm; 39009 may be used with 470-490nm CWL LEDs.
520
603
49005
39009
Ethidium homidimer-1/DNA 49005 may be used with LEDs with CWL 535-555nm; 39005 may be used with 535-550nm CWL LEDs.
527
617
49005
39005
EYFP/pH 7 49003, 39003 may be used with LEDs with CWL 490-505nm
514
527
49003
39003
F
FAM 49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.
492
518
49011
49002
39002
FITC 49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.
490
525
49011
49002
39002
FlAsH-CCPFCC 49003, 39003 may be used with LEDs with CWL 490-505nm.
511
530
49003
39003
Fluo-3 49003 may be used with LEDs with CWL 490-505nm; 49011 may be used with 465-495nm CWL LEDs.
505
526
49003
49011
Fluo-4 49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.
494
516
49011
49002
39002
FluoroGold This dye has mainly been used as a retrograde neuronal tracer, although it can also distinguish between DNA and RNA binding according to its fluorescence emission. With two excitation peaks at approx. 330nm and 390nm, and two emission peaks at approx. 450nm and 600nm, our DAPI longpass filter sets 49025 and 19012 are appropriate for detection of the fluorescence emission associated with the both peaks, which together appear "gold". The 49025 set is also useful for distinguishing between FluoroGold bound to DNA or RNA.
49025
19012
FM 1-43 39009, 19010 may be used with LEDs with CWL 470-490nm.
479
598
39009
19010
FM 4-64 For imaging applications using a monochrome camera, this longpass filter set will not distinguish FM 4-64 from TRITC or similar orange/red fluorochromes. To be able to distinguish between FM 4-64 and TRITC or Texas Red-like fluorochromes, for a custom filter set. 19010 may be used with LEDs with CWL 470-490nm; 19006 may be used with 545-575nm CWL LEDs.
515
740
19010
19006
Fura-2/Ca2+ - free
340
510
79001
Fura-2/Ca2+ - saturated
380
510
79001
FusionRed 49008, 39010 and 49017 may be used with LEDs with CWL 545-575nm.
576
603
49008
49017
39010
49910
49909
G
GFP EGFP spectra used here; similar to Emerald GFP, TagGFP, Azami Green, mWasabi, etc.49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.
488
507
49002
49020
39002
H
HEX,SE 49014 may be used with LEDs with CWL520-540nm; 49023 may be use with 485-515nm CWL LEDs.
533
559
49014
41023
Hoechst 33258 49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.
352
461
49000
39000
I
Indo-1/Ca2+ -free 79006 may be used with LEDs with CWL 365nm.
346
475
79006
indo-1/Ca2+ -saturated 79006 may be used with LEDs with CWL 365nm.
330
401
79006
Indocyanine Green The spectral properties of ICG are strongly dependent on its concentration and the solute it is bound to, so care must be taken by workers to characterize their particular model system. This filter set 49030 is appropriate for most applications involving protein-bound ICG in various physiological media. ICG becomes concentrated in lipid environments, greatly shifting its spectral properties. 49030 may be used with LEDs with CWL 755-795nm.
49030
J
JC-1 non-ratiometric JC-1 exists as a monomer at low concentrations and/or low membrane potentials and displays fluorescence ex./em. maxima similar to that of YFP. At higher concentrations and/or higher membrane potentials, JC-1 forms aggregates with a red-shifted emission maxima of 590nm and a broadened excitation spectra. The 49012 longpass filter set will allow detection of JC-1 monomer and aggregate simultaneously.
49012
19002
JOE 49023 may be use with LEDs with CWL 485-515nm.
520
548
49023
49905
K
Killer Red 49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.
585
611
49008
39010
49306
49911
49910
L
Li-Cor IRDye 680LT 49022 may be used with LEDs with CWL 635-665nm.
674
693
49022
Li-Cor IRDye 800CW 49037 may be used with LEDs with CWL 725-755nm.
778
794
49037
Lucifer Yellow 49026 may be used with LEDs with CWL 390-415nm; 39008 may be used with 405-430nm CWL LEDs
428
540
39008
49026
LysoTracker Blue/MeOH 49028 may be used with LEDs with CWL 385-400nm; 49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.
373
425
49000
39000
49028
LysoTracker Green/pH 5.2
504
511
49002
49020
39002
49904
LysoTracker Red/pH 5.2
577
590
49008
49909
LysoTracker Yellow HCK-123
486
563
39009
49023
M
mCherry
587
610
49008
49306
39010
49910
49911
mCitrine 49003, 39003 may be used with LEDs with CWL 490-505nm; 49023 may be use with 490-520nm CWL LEDs.
514
529
49003
39003
49905
49904
MitoTracker Deep Red 633/MeOH
644
665
49006
49009
39007
MitoTracker Green FM/MeOH
490
516
49002
49011
39002
MitoTracker Orange/MeOH
551
576
49004
49305
39004
MitoTracker Red/MeOH
578
599
49008
49306
39010
49910
mKate2
589
634
49008
39010
49306
49911
49912
mKeima Red
440
620
49024
mKO
548
559
49010
49014
49907
49906
mOrange2
548
562
49010
49014
49907
49906
mRFP1
584
607
49008
39010
49306
49910
49911
mTFP1
462
492
49013
49903
49902
mWasabi
493
509
49002
49020
39002
49904
N
NBD X/MeOH
467
538
49011
19008
Nile Blue
631
660
49006
39007
NirFP
604
669
49019
49912
O
Oregon Green 488
490
514
49011
49003
39002
Oregon Green 514 49003, 39003 may be used with LEDs with CWL 490-505nm.
506
526
49003
49303
39003
P
Pacific Blue
405
455
49021
19011
Propidium Iodide
536
617
49005
39005
Q
Qdot 525 Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
250-450
525
39101
Qdot 545 Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
250-450
545
39102
Qdot 565 Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
250-450
565
39103
Qdot 585 Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
250-450
585
39104
Qdot 605 Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
250-450
605
39105
Qdot 625 Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
250-450
625
39106
Qdot 655 Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
250-450
655
39107
Qdot 705 Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
250-450
705
39108
Qdot 800 Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
250-450
800
39109
R
R-phycoerythrin
565
578
49010
49014
19004
49909
ReAsH-CCPGCC
592
606
49008
39010
49306
Rhod-2 49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
552
581
49004
49305
39004
49908
Rhodamine 123 49003, 39003 may be used with LEDs with CWL 490-505nm.
507
529
49003
39003
49905
Rhodamine 6G 49023 may be use with LEDs with CWL 485-515nm.
525
555
49023
49906
49907
Rhodamine Red-X
570
590
49008
39010
49910
ROX
578
604
49008
39010
49910
S
SBFI/Na+ -free
340
505
79001
SBFI/Na+ -saturated
380
505
79001
Sulforhodamine 101
586
605
49008
39010
SYBR Green I
498
522
49002
49011
39002
49904
SYTO 9/DNA
483
500
49002
49011
39002
49904
SYTO 60
652
678
49006
49009
39007
49913
49914
T
TagBFP 49021 may be used with 400-410nm CWL LEDs, 49028 may be used with 385-400nm CWL LEDs
402
457
49021
49028
49901
TagRFP
556
584
49004
39004
49305
49909
49908
TAMRA
555
580
49004
49005
39004
49909
tdTomato
554
581
49004
49305
39004
49909
49908
TET
521
536
49023
Tetramethylrhodamine isothiocyanate
555
580
49004
49305
39004
49909
49908
Texas Red
595
620
49008
39010
49306
49911
49910
Texas Red-X
595
615
49008
39010
49306
TO-PRO-3
642
661
49006
39007
49009
Topaz 49003, 39003 may be used with LEDs with CWL 490-505nm.
514
529
49003
39003
49905
49904
TRITC
555
580
49004
49305
39004
49909
49908
TurboFP650
592
650
49008
19006
49017
49912
49911
W
wtGFP
475
509
49002
19008
X
X-rhod-1/Ca2+
580
601
49008
39010
49306
Z
ZsGreen1
493
505
49002
49020
39002
49904
ZsYellow
529
539
49023
49003
49905
39004
49305

 



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